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B8 Metabolische Prozessanalyse und -optimierung bakterieller Kultivierungen (Bacillus megaterium)

(Franco-Lara)

Publikationen/ Publications

2012

Korneli, C., David, F., Biedendieck, R.,Jahn, D. & Wittmann, C. (2012) The Big Beast at Work – Systems Biotechnology for Recombinant Protein Production in Bacillus megaterium J Biotechnol., in press

David F, Hebeisen M, Schade G, Franco-Lara E, Di Berardino M (2012) Viability and membrane potential analysis of Bacillus megaterium cells by impedance flow cytometry. Biotechnol. Bioeng. 109:483-492.

2011

1. David, F., Hebeisen, M., Schade, G., Franco-Lara, E., Di Berardino, M. (2011). Viability and membrane potential analysis of Bacillus megaterium cells by impedance flow cytometry. Biotech. Bioeng. In press.

2. David, F., Steinwand, M., Hust, M., Bohle, K., Ross, A., Dübel, S., Franco-Lara, E. (2011). Antibody production in Bacillus megaterium: strategies and physiological implications of scaling from micro titer plates to industrial bioreactors. Biotechnology J. In press.

3. Korneli, C., David, F., Godard, T., Franco-Lara, E. (2011). Influence of fructose and oxygen gradients on fed-batch recombinant protein production using Bacillus megaterium. Eng. Life Sci. In press.

4. Schädel, F., David, F., Franco-Lara, E. (2011) Evaluation of cell damage caused by cold sampling and quenching for metabolome analysis. Appl. Microbiol. Biotechnol. In Press.

5. Lueders, S., David, F., Steinwand, M., Jordan. E., Hust, M., Dübel, S., Franco-Lara, E. (2011). Influence of the hydromechanical stress and temperature on growth and antibody fragment production with Bacillus megaterium. Appl. Microbiol. Biotechnol. 91:81-90.

6. David, F., Berger, A., Hansch, R., Rohde, M., Franco-Lara, E. (2011). Single cell analysis applied to antibody fragment production with Bacillus megaterium: development of advanced physiology and bioprocess state estimation tools. Microb. Cell Fact. 10:23.

2010

David., F., Westphal, R., Bunk, B., Jahn, D., Franco-Lara, E. (2010). Optimization of antibody fragment production in Bacillus megaterium: the role of metal ions on protein secretion. J. Biotechnol. 150:115-124.

2009

Biedendieck, R., Bunk, B., Fürch, T., Franco-Lara, E., Jahn, M., Jahn, D. (2009). Systems Biology of Recombinant Protein Production in Bacillus megaterium. Chapter in “Systems Biotechnology.” Springer-Verlag Series “Advances in Biochemical Engineering/ Biotechnology 113.” (In Press)

Vorträge und Poster/ Conference Presentations

2009

David, F., Göcke, Y., Bewersdorf, T., Hellmers, F., Lüders, S., Jordan, E., Hust, M., Dübel, S., Franco-Lara, E. (2008). Bioprocess Design of Antibody Fragment-Secreting Bacillus megaterium MS941 Cultivations. 7th European Symposium on Biochemical Engineering Science (ESBES 7). Faro, Portugal, 7.-10. September, 2008

Franco-Lara, E., David, F., Lüders, S. (2008). Bioprocess design of the antibody fragment D1.3 scFv production. German-Mexican Workshop on the Integration of Microbial Physiology and Bioprocess Technology. 21.-23. September 2008, Cuernavaca, Mexico.

David, F., Korneli, C., Schön, M., Westphal, R., Göcke, Y., Franco-Lara, E. (2009). Bacillus megaterium: a microbial cell factory for antibody fragments. 12th German-American Frontiers of Engineering Symposium, 22.-25. April 2009, Potsdam, Germany.

Fürch, T., Franco-Lara, E., Jahn, D., Hempel, D. C., Wittmann, C. (2009). Genetic algorithms for modelling and optimization of recombinant protein production. Dechema Jahrestagung der Biotechnologen 2009, 8.09.-10.09.09, Mannheim, Germany.

David, F., Korneli, C., Schön, M., Westphal,R., Franco-Lara, E. (2009). Process optimization of antibody fragment-secreting Bacillus megaterium cultivations and its effect on the cell heterogeneity. New Biotechnology, Volume 25, Supplement 1, September 2009, Page S217. doi:10.1016/j.nbt.2009.06.174

David, F., Korneli, C., Schön, M., Berger, A., Franco-Lara, E. (2009). Culture heterogeneity in Bacillus megaterium cultivations producing antibody fragments regarding the state of production and membrane potential. 19th Annual Conference of the German Society for Cytometry. October 14-16, 2009. Helmholtz-Centre for Environmental Research, Leipzig, Germany.

David, F., Korneli, C., Franco-Lara, E. (2009). Cell physiology and heterogeneity in the production of antibody fragments. BioProScale Symposium: Inhomogeneities in large scale bioreactors. November 24-27, 2009. Berlin, Germany.


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