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B1 Heterologe Proteinproduktion mittels rekombinanter Mikroorganismen

(Rinas)

(1. Förderphase) , in 2. Förderphase in B4 überführt.

Publikationen/ Publications

 

2005

Wendler, J., Vallejo, L. F., Rinas, U., Bilitewski, U. (2005). Application of an SPRbased receptor assay for the determination of biological active recombinant bone morphogenetic protein-2. Anal. Bioanl. Chem. , 381, 1056-1064

Rinas, U., El-Enshasy, H., Emmler, M., Hille, A., Hempel, D. C., Horn, H. (2005). Model-based prediction of substrate conversion and protein synthesis and
excretion in recombinant Aspergillus niger biopellets
. Chem. Eng. Sci., 60, 2729-2739

Kadow, S., Betiku, E., Rinas, U., Bilitewski, U. (2005). Development of a rapid, quantitative glucosyltransferase assay based on a screen-printed fructose enzyme electrode and application to optimization studies on gtfD expression in recombinant Escherichia coli. Biotechnol. Bioeng., 91, 454-461

2004

Vallejo, L.F. and Rinas, U. (2004). Strategies for the recovery of active proteins through refolding of bacterial inclusion body proteins. Microb. Cell Fact., 3, 11

Hoffmann, F., Rinas, U. (2004). Roles of heat-shock chaperones in the production of recombinant proteins in Escherichia coli. Adv. Biochem. Eng. Biotechnol., 89, 143-161 (2004)

Hoffmann, F., Rinas, U. (2004). Stress induced by recombinant protein production in Escherichia coli. Adv. Biochem. Eng. Biotechnol., 89, 73-92

Rinas, U., Hoffmann, F. (2004). Leakage of DnaK and other osmotic shock sensitive proteins in high-cell density cultures of Escherichia coli. Biotechnol. Prog., 20, 679-687 (2004)

Vallejo, L.F., Rinas, U. (2004). Optimized procedure for renaturation of recombinant human bone morphogenetic protein-2 at high protein concentration. Biotechnol. Bioeng., 85, 601-609 (2004)

2003

Carrió, M.M., Villaverde, A. (2003). Role of molecular chaperones in inclusion body formation. FEBS Lett. 537, 215-221

2002

Vallejo, L. F., Brokelmann, M., Marten, S., Trappe, S., Cabrera-Crespo, J., Hoffmann, A., Gross, G., Weich, H. A., Rinas, U. (2002). Renaturation and purification of bone morphogenetic protein-2 produced as inclusion bodies in high-cell-density cultures of recombinant Escherichia coli. J. Biotechnol. 94, 185-194

Vallejo, L.F., Rinas, U. (2002). Strategies for refolding inclusion body proteins. Villaverde, A. (ed.) Protein production in bacterial cell factories. Research Signpost, Trivandrum-695 023, Kerala, India, pp. 59-71

Carbonell, X., Villaverde, A. (2002). Protein aggregated into bacterial inclusion bodies does not result in protection from proteolytic digestion. Biotechnol. Lett. 24, 1939-1944

 

Buchveröffentlichungen/ Released Books

Vallejo, L.F. and Rinas, U. (2002). Strategies for refolding inclusion body proteins. Villaverde, A. (ed.) Protein production in bacterial cell factories. Research Signpost, Trivandrum-695 023, Kerala, India, pp. 59-71

 

Vorträge und Poster/ Conference Presentations

 

2004

Lu, X., Ganzlin, M. and Rinas, U. (2004). A proteomic approach to study cell physiology during glaA promotor controlled heterologous gene expression in recombinant Aspergillus niger. 3rd International Conference on Recombinant Protein Production, November 11-14, Tavira, Algarve, Portugal

Betiku, E., Strebe, N., Hofer, B. and Rinas, U. (2004). Extracellular production of glucosyltransferase D in Escherichia coli. 3rd International Conference on Recombinant Protein Production, November 11-14, Tavira, Algarve, Portugal

Gueorguieva, L., Seidel-Morgenstern, A., Vallejo, L.F. and Rinas, U. (2004). Continuous separation of the monomeric and dimeric forms of human bone morphogenetic protein-2 (BMP-2) using gradient simulated moving bed (SMB) chromatography. Poster No. 44. International Symposium on the Separation of Proteins, Peptides and Polynucleotides (ISPPP). October 19-22, Aachen

Gueorguieva, L., Vallejo, L.F., Rinas, U., Seidel-Morgenstern, A. (2004). Einfluß des Salzgehaltes auf die Adsorptionsgleichgewichte und das Elutionsverhalten der Monomeren und Dimeren des humanen Knochenwachstumsfaktors BMP-2. BioPerspectives. 4-6 Mai, Wiesbaden

Lu, X., Ganzlin, M. and Rinas, U. (2004). A proteomic approach to study glaA promotor controlled heterologous gene expression in recombinant Aspergillus niger. 121th Event of the European Federation of Biotechnology, Physiology of Yeasts and Filamentous Fungi (PYFF2: Second Meeting), March 24-28, Anglet, France

2003

Vallejo, L.F., Rinas, U. (2003). Optimized procedure for renaturation of recombinant human bone morphogenetic protein-2 at high protein concentration. 11th European Congress on Biotechology, Poster 599, Basel, Switzerland

Horn, H., Hille, A., Kelly, S., Grimm, L., Hengstler, J., Emmler, M., Jungebloud, A., Cordes, C., Krull, R., Hempel, D. C., Jahn, D., El-Enshasy, H., Rinas, U. (2003). Einfluss der Pelletmorphologie von Aspergillus niger auf Stoffumsatz und Produktbildung. Experimentelle Untersuchungen und Modellierung
GVC/Dechema-Jahrestagung, Mannheim, Chem.-Ing.-Tech. 75, 1070-1071

 

Patente/ Patents

Vallejo, F., Rinas U. (2003). Method for the production of cystine knot proteins. (European Patent Application 03 00 3219.7)

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