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A3 Erzeugung, Screening und Charakterisierung modifizierter Glycosyltransferasen

(Buchholz/Hofer)

Publikationen/ Publications

2008.

Wittrock, S., Swistowska,  A. M., Collisi, W., Hofmann, B., Hecht, H.-J., Hofer, B. (2008). Re- or displacement of invariant residues in the C-terminal half of the catalytic domain strongly affects catalysis by glucosyltransferase R. FEBS Lett. 582, 491–496.

Swistowska, S., Wittrock, S., Collisi, W., Hofer, B. (2008). Heterologous hyper-expression of a glucansucrase type glycosyltransferase gene. Appl. Microbiol. Biotechnol. 79, 255-261.

Hellmuth, H., Wittrock, S., Kralj, S., Dijkhuizen, L., Hofer, B., Seibel, J. (2008). Engineering the Glucansucrase GTFR Enzyme Reaction and Glycosidic Bond Specificity: Toward Tailor-Made Polymer and Oligosaccharide Products. Biochemistry 47, 6678–6684.

2007

Zuccaro, A., Götze, S., Kneip, S., Dersch, P., Seibel, J. (2007). Taylor made fructooligosaccharides by combination of substrate and genetic engineering. ChemBioChem, im Druck

Kralj, S., Buchholz, K., Dijkhuizen, L., Seibel, J. (2007). Fructansucrase enzymes and sucrose analogues: new approach for the synthesis of unique fructo-oligosaccharides. Biocatal. Biotrans, im Druck

Swistowska, A.M., Gronert, S., Wittrock, S., Collisi, W., Hecht, H.J., Hofer, B. (2007). Identification of structural determinants for substrate binding and turnover by glucosyltransferase R supports the permutation hypothesis. FEBS Lett., 581(21), 4036-4042

Hellmuth, H., Hillringhaus, L., Höbbel, S., Kralj, S., Dijkhuizen, L., Seibel, J. (2007). Highly efficient Chemoenzymatic Synthesis of Novel branched Thiooligosaccharides via Substrate Direction using Glucansucrases.ChemBioChem, 8, 273-276

Biedendieck, R., Beine, R., Gamer, M, Jordan, E, Buchholz, K., Seibel, J., Dijkhuizen, L., Malten, M., Jahn, D. (2007). Export, purification and activities of affinity tagged Lactobacillus reuteri levansucrase produced by Bacillus megaterium. Appl Microbiol Biotechnol, 74(5), 1062-1073 (2007)

Swistowska, A.M., Gronert, S., Wittrock, S., Collisi, W., Hecht, H.-J., Hofer, B. (2007).Identification of structural determinants for substrate binding and turnover by glucosyltransferase R supports the permutation hypothesis. FEBS Lett. 581, 4036-4042

2006

Seibel, J. (2006). Enzymatische Oligosaccharidsynthesen: vom Gen zum Produkt. Nachrichten aus der Chemie, 2, 110-114

Seibel, J., Jördening, H.-J., Buchholz, K. (2006). Glycosylation with activated sugars using glycosyltransferases and transglycosidases. Biocat. Biotrans., 24, 311-354

Seibel, J., Hellmuth, H., Hofer, B., Schmalbruch, B. (2006). Identification of new donor specificities of Glycosyltransferase R via the aid of substrate microarrays. ChemBioChem, 7, 310-320

Pohl, W., Hellmuth, H., Hilbert, M., Seibel, J., Walla, P.J. A two-photon fluorescence correlation study of lectins interacting with carbohydrated 20 nm beads. ChemBioChem, 7, 268-274

Seibel, J., Moraru, R., Götze, S., Na’amnieh, S., Pawlowski, A., Buchholz, K. (2006). Synthesis of sucrose analogues and the mechanism of action of Bacillus subtilis fructosyltransferase (levansucrase). Carbohydr. Res., 341, 2335-2349

Seibel, J., Beine, R., Moraru, R., Behringer, C., Buchholz, K. (2006). A New Pathway for the Synthesis of Oligosaccharides by the Use of non-Leloir glycosyltransferases. Biocat. .Biotrans.., 24, 157-165

Seibel, J. (2006). Enzymatische Oligosaccharidsynthesen: vom Gen zum Produkt. Nachrichten aus der Chemie, 2, 110-114

Seibel, J, Hellmuth, H., Hofer, B., Kicinska, A.-M, Schmalbruch, B. (2006). Identification of New Acceptor Specificities of Glycosyltransferase R with the Aid of Substrate Microarrays. J ChemBioChem. 7, 310-320

2005

Seibel, J., Beine, R., Moraru, R., Behringer, C., Buchholz, K. (2005). A new pathway for the synthesis of oligosaccharides by the use of non-Leloir glycosyltransferases.

Seibel, J., Beine, R., Moraru, R., Nimtz, M., Buchholz, K. (2005). A new enzymatic route for oligosaccharide synthesis utilizing sucrose as an energy source

Baciu, I.E., Jördening, H.-J., Seibel, J., Buchholz, K. (2005). Investigations of the transfructosylation reaction by fructosyltransferase from B. subtilis NCIMB 11871 for the synthesis of sucrose analogue galactosyl-fructoside. J. Biotechnol. , 116347-357

Baciu, I.-E., Jördening, H.-J., Seibel, J., Buchholz, K. (2005). Characterisation of a transfructosylation reaction for the synthesis of sucrose analogue. J. Biotechnology

Vorträge und Poster/ Conference Presentations

2007

Buchholz, K. (2007). Industrial carbohydrate biotransformations (einschließlich einer kurzen Darstellung unserer jüngsten Ergebnisses aus SFB-Projekten). EUROCARB 14, Lübeck

Buchholz, K. (2007). A New Pathway for the Synthesis of Oligosaccharides by Glycosyltransferases. Industrial Biocatlysis, Osaka

Buchholz, K. (2007). Highlights in Biocatalysis – Historical Landmarks and Current Trends. Achema, Frankfurt

Beine, R., Tyralla, B., Seibel, J. (2007). Production of sucrose analogues via combination of a biocatalytic with a fermentative process. European BioPerspectives, Köln

Hillringhaus, L., Hellmuth, H., Höbbel, S., Seibel, J. (2007). Chemoenzymatic synthesis of branched thioglycosides. European BioPerspectives, Köln

Hellmuth, H., Wittrock, S., Hofer, B., Seibel, J. (2007). GTFR-Variants of streptococcus oralis show different polymer- and acceptor specificities. European BioPerspectives, Köln

Seibel, J., Hellmuth, H., Hillringhaus, L., Moraru, R., Götze, S., Beine, R. (2007). Neuartige enzymatische Glykosylierungsmethoden zur Synthese von Oligosacchariden, Glykopeptiden und Glykokonjugaten. 40. Jahrestreffen Deutscher Katalytiker, Weimar

Pohl, W., Hellmuth, H., Hilbert, M., Walla, P.J., Seibel, J. (2007). A two-photon fluorescence correlation study of lectins  interacting with carbohydrated 20 nm beads. CBM7, Braunschweig

Hellmuth, H. Beine, R., Kralj, S. Dijkhuizen, L., Seibel, J. (2007). Enzyme- and Substrate-directed synthesis of oligosaccharides and glycoconjugates. CBM7, Braunschweig

Beine, R., Moraru, R. Nimtz, M. Buchholz, K. Seibel, J. (2007). Sucrose analogues - novel substrates for the fructosyltransferase of B. subtilis NCIMB 11871. CBM7, Braunschweig

Seibel, J., Hoebbel, S., Hillringhaus, L. (2007). Chemoenzymatic synthesis of thiooligosaccharides. CBM7, Braunschweig

Schneider, J., Hofer, B. (2007). High-level expression of a recombinant amylosucrase gene and turnover of sucrose analogues by the enzyme. 32nd Annual FEBS Congress**, Wien, 2007.

2006

Seibel, J., Hellmuth, H., Hillringhaus, L., Moraru, R., Beine, R., Götze, S. (2006). Novel Chemoenzymatic Pathways to Oligosaccharides, Glycopeptides and Glycoconjugates. biocat2006, Hamburg-Harburg

Hellmuth, H., Hillringhaus, L., Höbbel, S., Dijkhuizen, L., Krajl, S., Seibel, J. (2006).Enzymatic Synthesis of Novel Oligosaccharides and Glycostructures by different Glycosyltransferases. biocat2006, Hamburg-Harburg

2005

Seibel, J,., Moraru, R., Götze, S., Jördening, H.-D., Na’amnieh, S., Hillringhaus, L. (2005). Enzymatic, chemoenzymatic and chemical synthesis of sucrose analogues. BioPerspectives, Wiesbaden (2005)

Buchholz, K., Seibel, J,., Baciu, I. - E., Beine, R., Jördening, H.-D. (2005). A pathway for the synthesis of oligosaccharides by the use of non-Leloir glycosyltransferases. 6th Carbohydrate Bioengineering meeting (CBM6), Barcelona

Seibel, J., Hellmuth, H., Hillringhaus, L., Morarou, R., Götze, S. (2005). Chemoenzymatic tools for the synthesis of oligosaccharides, glycopeptides and glycoconjugates. 6th Carbohydrate Bioengineering meeting (CBM6), Barcelona

Seibel, J., Moraru, R., Hillringhaus, L., Okoro, L. (2005). Chemical and Enzymatic Methods for the Glycosylation and Glycoconjugation of Natural Products. First Austrian-German-Italian Meeting of Organic Chemistry, Vienna

Seibel, J., Götze, S., Moraru, R. (2005). Synthesis and isolation of novel fructooligomers. GVC/Dechema, Systembiotechnologie für industrielle Prozesse, Braunschweig

Seibel, J., Hellmuth, H., Hillringhaus, L. (2005). Glycoconjugation techniques for the identification of protein-carbohydrate interactions. GVC/Dechema, Systembiotechnologie für industrielle Prozesse, Braunschweig

2004

Kicinska, A. M., Wittrock, S., Gronert, S., Collisi, W., Hecht, H.-J., Hofer, B. (2004). Identification of structural determinants that are crucial for substrate binding by glycosyltransferases of the glucansucrase type. VAAM-Jahrestagung, Braunschweig

Seibel, J., Beine, R., Hofer, B., Hecht, K., Buchholz, K. (2004). Screening of transferases for the Production of New Oligosaccharides. biocat2004, Hamburg-Harburg

Seibel, J., Götze, S., Moraru, R. (2004). The Power of Enzymes in Oligosaccharide Synthesis. Biocat2004, Hamburg

Seibel, J., Beine, R., Hofer, B., Hecht, K., Buchholz, K. (2004). Screening of transferases for the production of new oligosaccharides. biocat2004, Hamburg-Harburg

2003

Baciu, I.-E., Jördening, H.-J., Buchholz, K. (2003). Screening of new glycosyltransferases for the production of oligosaccharides. ACHEMA-Tagung. 27th International Exhibition-Congress on Chemical Engineering, P. 11

Baciu, I.-E., Jördening, H.-J., Buchholz, K. (2003). Screening neuer Glycosyltransferasen zur Produktion von Oligosacchariden. 5. Veranstaltung der Vortragsreihe „Angewandte Umweltbiotechnologie aus Niedersachsen“ der Fachgruppe Umwelt des Life-Science-Netzwerkes Niedersachsen: Biokonversion nachwachsender Rohstoffe, Braunschweig

2001

Baciu, I.-E., Jördening, H.-J., Buchholz, K. BioTrans 2001, Darmstadt. 5th International Symposium on Biocatalysis and Biotransformation (2001)

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