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A1 Produktion rekombinanter Glycosyltransferasen in Aspergillus niger und Bacillus megaterium

(Jahn/Dersch)

Publikationen/ Publications

2013

Korneli, C., David, F., Biedendieck, R., Jahn, D. & Wittmann, C. (2013) Getting the big beast to work-systems biotechnology of Bacillus megaterium for novel high-value proteins, J Biotechnol 163:87-96

Korneli, C., Biedendieck, R., David, F., Jahn, D. & Wittmann, C. (2013) High yield production of extracellular recombinant levansucrase by Bacillus megaterium. Appl Microbiol Biotechnol 97:3343-3353

2012

Klein, J., Leupold, S., Biegler, I., Biedendieck, R., Münch, R. & Jahn, D. (2012) TLM-Tracker: Software for cell segmentation, tracking and lineage analysis in time-lapse microscopy movies. Bioinformatics, in press

2011

Eppinger, M., Bunk, B., Johns, M.A., Edirisinghe, J.N., Kutumbaka, K.K., Koenig, S.S.K., Creasy, H.H., Rosovitz, M.J., Riley, D.R., Daugherty, S., Martin, M., Elbourne, L.D.H., Paulsen, I., Biedendieck, R., Braun, C., Grayburn, Dhingra, S., Lukyanchuk, V., Ball, B., ul-Qamar, R., Seibel, J., Bremer, E., Jahn, D., Ravel, J. & Vary, P.S. (2011). Genome sequences of the biotechnologically important B. megaterium strains. QM B1551 and DSM319 J Bacteriol., 193: 4199-4213

Biedendieck, R., Borgmeier, C., Bunk, B., Stammen, S., Scherling, C., Meinhardt, F., Wittmann, C. & Jahn, D. (2011). Systems biology of recombinant protein production using Bacillus megaterium. Methods Enzymol 500: 165-195

Borgmeier, C., Biedendieck, R., Hoffmann, K., Jahn, D. & Meinhardt, F. (2011). Transcriptome profiling of degU expression reveals unexpected regulatory patterns in Bacillus megaterium and discloses new targets for optimizing expression. Appl Microbiol Biotechnol., DOI 10.1007/s00253-011-3575-x

Driouch, H., Roth, A., Dersch, P. and Wittmann, C. (2011) Filamentous fungi in good shape: Microparticles for tailor-made fungal morphology and enhanced enzyme production. Bioengineered Bugs 2:2, 1-5.

Driouch, H., Roth, A., Dersch, P. and Wittmann, C. (2010) Optimized bioprocess for production of fructofuranosidase  by recombinant Aspergillus niger.  Appl. Microbiol. Biotechnol. 87: 2011-2024.

Strube, C.P., Homann, A., Gamer, M., Jahn, D., Seibel, J. & Heinz, D.W. (2011) Polysaccharide synthesis of the levansucrase SACB from Bacillus megaterium is controlled by distinct surface motifs. J Biol Chem 286: 17593-17600

 

2010

Fleißner, A. and Dersch, P. (2010) Expression and Export: Recombinant protein production systems for Aspergillus. Appl. Microbiol. Biotechnol. 87: 1255-1270.

Stammen, S., Schuller, F., Dietrich, S., Gamer, M., Biedendieck, R. & Jahn, D. (2010) Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium. Appl Microbiol Biotechnol 88: 529-539

Biedendieck, R., Bunk, B., Fürch, T., Franco-Lara, E., Jahn, M. & Jahn, D. (2010) Systems Biology of Recombinant Protein Production in Bacillus megaterium in "Advances in Biochemical Engineering / Biotechnology" (Scheper, T., Ed.); Vol. 113 - Biosystems Engineering (Wittmann, C., Ed.) Springer, Berlin, Heidelberg 120:133-161

Bunk, B., Biedendieck, R., Jahn, D. & Vary, P.S. (2010) Bacillus megaterium and other Bacilli: Industrial Applications. In Flickinger M. C. (Ed.) Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology. Volume 1 pp 429-443. John Wiley & Sons. Inc., Hoboken, NJ.

Roth, A. and Dersch, P. (2010) A novel expression system for intracellular production and purification of recombinant affinity-tagged proteins in Aspergillus niger. Appl. Microbiol. Biotechnol. 86: 659-670.

Homann A., Ul-Qamar R. Serim S., Dersch P., and J. Seibel (2010). Bioorthogonal metabolic glycoengineering of human larynx carcinoma (HEp-2) cells targeting sialic acid. Beilstein J Org Chem. 2010 Mar 8;6:24.

Stammen, S., Müller, B.K., Korneli, C., Biedendieck, R., Gamer, M., Franco-Lara, E. & Jahn, D. (2010). High yield intra- and extracellular protein production using Bacillus megaterium. Appl. Environ. Microbiol. 76: 4037-4046

Bunk, B., Schulz, A., Stammen, S., Münch, R., Warren, M. J., Rohde,  M., Jahn, D., Biedendieck, R. (2010). A short story about a big magic bug. Bioengineered Bugs 1: 1-7

Biedendieck, R., Malten, M., Barg, H., Bunk, B., Martens, J.-H.,  Deery, E., Leech, H., Warren, M. J., Jahn, D. (2010). Metabolic engineering of cobalamin (vitamin B12) production in  Bacillus megaterium. Microbial Biotechnology 3: 24-37

2009

Beine, R., Valente, A.R., Biedendieck, R., Jahn, D., Seibel, J. (2009). Directed optimization of biocatalytic transglycosylation processes  by the integration of genetic algorithms and fermentative  approaches into a kinetic model. Process Biochemistry 44: 1103–1114

Gamer, M., Froede, D., Biedendieck, R., Stammen, S., Jahn, D. (2009). A T7 RNA polymerase-dependent gene expression system for Bacillus megaterium. Appl Microbiol Biotechnol. 82:1195-203.

2008

Zuccaro, A., Gotze, S., Kneip, S., Dersch, P., Seibel, J. (2008). Tailor-made fructooligosaccharides by a combination of substrate and genetic engineering Chembiochem 9(1):143-9.

2007

Biedendieck, R., Beine, R., Gamer, M., Jordan, E., Buchholz, K., Seibel, J., Dijkhuizen, L., Malten, M., Jahn, D. (2007) Export, purification and activities of affinity tagged Lactobacillus reuteri levansucrase produced by Bacillus megaterium. Appl Microbiol Biotechnol. 74:1062-1073.

Biedendieck, R., Yang, Y., Deckwer, W.-D., Malten, M., Jahn, D. (2007). Plasmid system for the intracellular production and purification of affinity-tagged proteins in Bacillus megaterium. Biotechnol Bioeng. 96:525-537.

Biedendieck, R., Gamer, M., Jaensch, L., Meyer, S., Rohde, M., Deckwer, W.-D., Jahn, D. (2007). A sucrose-inducible promoter system for the intra- and extracellular protein production in Bacillus megaterium. J Biotechnol. 132:426-430.

Homann, A., Biedendieck, R., Goetze, S., Jahn, D., Seibel, J. (2007). Insights into polymer versus oligosaccharide synthesis - Mutagenesis and mechanistic studies of a novel levansucrase from Bacillus megaterium. Biochem J. 407:189-198.

Bäumchen, C., Roth, A. H. F. J., Biedendieck, R., Malten, M., Follmann, M., Sahm, H., Bringer-Meyer, S., Jahn, D. (2007). D-Mannitol production by resting state whole cell biotransformation of D-fructose by heterologous mannitol and formate dehydrogenase genes expression in Bacillus megaterium. Biotechnol J. 2:1408-1416.

Vary, P. S., Biedendieck, R., Fuerch, T., Meinhardt, F., Rohde, M., Deckwer, W.-D., Jahn, D. (2007). Bacillus megaterium – from simple soil bacterium to industrial protein production host. Appl Microbiol Biotechnol. 76:957-967. Review.

Jordan, E., Hust, M., Roth, A., Biedendieck, R., Schirrmann, T., Jahn, D., Dübel, S. (2007). Production of recombinant antibody fragments in Bacillus megaterium. Microb Cell Fact. 6:2.

Yang, Y., Malten, M., Grote, A., Jahn, D., Deckwer, W.-D.Codon. (2007). Optimized Thermobifida fusca hydrolase secreted by Bacillus megaterium. Biotechnol Bioeng. 96:780-794.

2006

Yang, Y., Biedendieck, R., Wang, W., Gamer, M., Malten, M., Jahn, D., Deckwer, W. D. (2006). High yield recombinant penicillin G amidase production and export into the growth medium using Bacillus megaterium. Microb Cell Fact.5:36.

Hiller, K., Grote, A., Maneck, M., Muench, R., Jahn, D. (2006). JVirGel 2.0: computational prediction of proteomes separated via two dimensional gel electrophoresis under consideration of membrane and secreted proteins. Bioinformatics 22:2441-2443.

Scheer, M., Klawonn, F., Muench, R., Grote, A., Hiller, K., Choi, C., Koch, I., Schobert, M., Haertig, E., Klages, U., Jahn, D. (2006). JProGO: A novel tool for the functional interpretation of prokaryotic microarray data using Gene Ontology information. Nucleic Acids Res. 34:510-515.

Malten, M., Biedendieck, R., Gamer, M., Buchholz, K., Dijkhuizen, L., Jahn, D. (2006). Bacillus megaterium plasmid system for the high level production, export and one-step purification of affinity tagged levansucrase from the growth medium. Applied Environ Microbiol. 72:1677-1679.

Hollmann, R., Malten, M., Biedendieck, R., Yang, Y., Wang, W., Jahn, D., Deckwer, W.-D. (2006). Bacillus megaterium als Produktionssystem für rekombinante Proteine. Chemie Ingenieur Technik 78:289-294.

2005

Wang, W., Hollmann, R., Fuerch, T., Nimtz, M., Malten, M., Jahn, D., Deckwer, W.-D. (2005). Proteome analysis of a recombinant Bacillus megaterium strain during heterologous production of a glucosyltransferase. Proteome Sci. 3, 4.

Malten, M., Nahrstedt, H., Meinhardt, F., Jahn, D. (2005). Coexpression of the type I signal peptidase gene sipM increased recombinant protein production and export in Bacillus megaterium. MS941 Biotechnol Bioeng. 91:616-621.

Malten, M., Hollmann, R., Deckwer, W.-D., Jahn, D. (2005). Production and secretion of recombinant Leuconostoc mesenteroides dextransucrase DsrS in Bacillus megaterium. Biotech Bioeng. 89:206-218.

2004

Hiller, K., Grote, A., Scheer, M., Muench, R., Jahn, D. (2004). PrediSi: prediction of signal peptides and their cleavage positions. Nucleic Acids Res. 32:375-379.

2003

Leech, H. K., Raux, E., Mclean, K. J., Munro, A. W., Robinson, N. J., Borrelly, G. P., Malten, M., Jahn, D., Rigby, S. E., Heathcote, P., Warren, M. J. (2003). Characterization of the cobaltochelatase CbiXL from Bacillus megaterium: evidence for 4Fe-4S center housed within an MXCXXC motif. J Biol Chem. 278:41900-41907.

Raux, E., Leech, H. K., Beck, R., Schubert, H., Santander, P. J., Roessner, C. A., Scott, A. I., Thermes, C., Rambach, A., Martens, J.-H., Jahn, D., Warren, M. J. (2003). Identification and functional analysis of enzymes required for precorrin-2 dehydrogenation and metal ion insertion in the biosynthesis of sirohaem and cobalamin in Bacillus megaterium. Biochem J. 370:505-516.

2002

Martens, J.-H., Barg, H., Warren, M. J., Jahn, D. (2002). Microbial production of Vitamin B12. Appl Microbiol Biotechn. 58:275-285.

 

Buchveröffentlichungen/Released Books

Barg, H., Malten, M., Jahn, M., Jahn, D. (2005). Protein and vitamin production in Bacillus megaterium. In Methods in Biotechnology - Microbial products and biotransformations. (J. L. Barredo, ed.) Humana Press, 18:165-184.

 

Vorträge und Poster/Conference Presentations

2011

R. Biedendieck, S. Stammen, M. Gamer, C. Korneli, E. Franco-Lara, D. Jahn. (2011) Different Promoter Systems for the Rrecombinant Protein Production, Secretion and Purification using Bacillus megaterium. Prokagenomics, Göttingen, Germany, 18.-21.09.2011 (Poster)

C. Finger, M. Gamer, B. Bunk, T. Knuuti, D. Jahn, R. Biedendieck. (2011). Development of a codon-plus Bacillus megaterium strain for recombinant protein production. Prokagenomics, Göttingen, Germany, 18.-21.09.2011 (Poster)

T. Knuuti, M. Gamer, S. Stammen, D. Fröde, C. Finger, D. Jahn, R. Biedendieck. (2011). The role of ComK - First insights into development of natural competence in Bacillus megaterium?. Prokagenomics, Göttingen, Germany, 18.-21.09.2011 (Poster)

Stammen, S., Schuller, F.,  Dietrich, S., Wienecke, S., Knuuti, T., Finger, C., Jahn, D., Biedendieck, R. (2011). In vitro characterization of Escherichia coli phage K1E RNA polymerase and its in vivo application for protein production in Bacillus megaterium. VAAM-Jahrestagung, Karslruhe, Germany (Poster).

Stammen, S., Müller, B. K., Korneli, C., Knuuti, T., Finger, C., Franco-Lara, E., Jahn, D. and Biedendieck, R.. (2011). High yield intra- and extracellular protein production using Bacillus megaterium. BACELL-meeting, Göttingen, Germany, 14.-15.03.2011 (Vortrag)

2010

Simon Stammen, Franziska Schuller, Sylvia Dietrich, Sarah Wienecke, Tobias Knuuti, Constanze Finger, Dieter Jahn and Rebekka Biedendieck In vitro characterization of Escherichia coli phage K1E RNA polymerase and its in vivo application for protein production in Bacillus megaterium; VAAM-Jahrestagung, Karlsruhe, Germany, 03 - 06/04/2011.

Simon Stammen, Franziska Schuller, Sylvia Dietrich, Sarah Wienecke, Martin Gamer, Rebekka Biedendieck and Dieter Jahn. In vitro characterization of Escherichia coli phage K1E RNA polymerase and its in vivo application for protein production in Bacillus megaterium; Microbes and Industrial Biotechnology Bielefeld, Germany, 21-24 November 2010  

Stammen, S, Müller, B. K., Korneli, K., Biedendieck, R., Gamer, M., Franco-Lara E. & Jahn D. High-yield intra- and extracellular protein production using Bacillus megaterium, Microbes and Industrial Biotechnology Bielefeld, Germany, 21-24 November 2010  

2009

Stammen, S., Gamer, M., Müller, B.K., Schwerk, J., Schlums, H., Jahn, D. (2009). A plasmid borne system for high level protein production in Bacillus megaterium. VAAM-Jahrestagung, Bochum, Germany (Poster).

Fähte C., Melzer J., Roth A. H. F. J., Stammen S., Dersch P., Jahn D. (2009).  Klonierung und Expression von Glycosyltransferasen. Klausurtagung Sonderforschungsbereich578, St. Andreasberg im Harz, Germany 16 - 17/03 (Vortrag).

Roth A. H. F. J., Dersch P. (2009). A vector system for production and purification of recombinant proteins in Aspergillus niger. VAAM-Jahrestagung, Bochum, Germany 08 - 11/03 (Poster).

2008

Stammen, S., Gamer, M., Müller, B.K., Schwerk, J., Schlums, H., Jahn, D. (2008). A plasmid borne system for high level protein production in Bacillus megaterium. Recombinant Protein Production (RPP), Alghero, Italy (Poster).

Roth A. H. F. J., Dersch P. (2008). An alternative inducible protein expression system for Aspergillus niger. Recombinant Protein Production (RPP), Alghero, Italy, 24 - 28/08 (Poster).

Gamer, M., Bendig, C., Yang, Y., Stammen, S., Jahn, D. (2008). Development of a codon-plus Bacillus megaterium strain for the recombinant protein production VAAM-Jahrestagung, Frankfurt, Germany.

Fröde, D., Gamer, M., Stammen, S., Jahn, D. (2008). Development of a T7 RNA polymerase dependet gene expression system for Bacillus megaterium. VAAM-Jahrestagung, Frankfurt, Germany.

Stammen, S., Gamer, M. Schwerk, J., Schlums, H., Jahn, D. (2008). Construction of strong inducible promoters for Bacillus megaterium expression vectors VAAM-Jahrestagung, Frankfurt, Germany.

2007

Roth, A. H. F. J., Zuccaro A., Kneip S., Dersch P. (2007). Characterisation of a new inducible promoter for protein expression in Aspergillus niger, using a green fluorescent protein reporter system. 13th European Congress on Biotechnology, Barcelona, Spain

Gamer, M., Stammen, S., Biedendieck, R., Yang, Y., Fröde, D., Bendig, C., Jahn, D. (2007). Bacillus megaterium: An alternative expression system. European Congress on Biotechnology, Barcelona, Spain (2007)

2006

Biedendieck, R., Malten, M., Gamer, M., Jahn, D. (2006). High level production and export of recombinant proteins into the growth medium using Bacillus megaterium. Society for Industrial Microbiology - Annual Meeting, Baltimore, MD, USA

Biedendieck, R., Gamer, M., Malten, M., Jahn, D. (2006). Tools for the intracellular production and purification of affinity tagged proteins using Bacillus megaterium. Society for Industrial Microbiology - Annual Meeting, Baltimore, MD, USA

Biedendieck, R., Malten, M., Gamer, M., Jordan, E., Beine, R., Buchholz, K., Seibel, J., Dijkhuizen, L., Jahn, D. (2006). Bacillus megaterium: Tools for protein production, secretion and purification of affinity tagged proteins. VAAM-Jahrestagung, Jena, Germany

Biedendieck, R., Malten, M., Gamer, M., Jahn, D. (2006). Bacillus megaterium: Tools for the intracellular protein production and purification of affinity tagged proteins. VAAM-Jahrestagung, Jena, Germany

2005

Biedendieck, R., Gamer, M., Stammen, S., Jahn, D., Malten, M. (2005). High level production, export and purification of affinity tagged heterologous proteins using Bacillus megaterium. Proc. GVC/DECHEMA-Vortrags- und Diskussionstagung „Systembiotechnologie für industrielle Prozesse“, Braunschweig, Germany

Biedendieck, R., Gamer, M., Jahn, D., Malten, M. (2005). Bacillus megaterium – a versatile tool for production and secretion of heterologous proteins. 12th European Congress on Biotechnology, Copenhagen, Denmark

2004

Malten, M., Biedendieck, R., Drews, A.-C., Hagemeister, J., Jahn, D. (2004). Bacillus megaterium - a versatile tool for production and secretion of heterologous proteins. 3rd Recombinant Protein Production Meeting, Tavira, Portugal

Riessen, S., Malten, M., Jahn, D., Antranikian, G. (2004). Cloning and expression of a keratin-hydrolysing protease of Thermoanaerobacter keratinophilus in Bacillus megaterium. Biocat, Hamburg.

Malten, M., Biedendieck, R., Drews, A.-C., Gamer, M., Jahn, D. (2004). Production and secretion of glycosyltransferases in Bacillus megaterium. Glycosciences, Wageningen, The Netherlands.

Malten, M., Biedendieck, R., Drews, A.-C., Hagemeister, J., Jahn, D. (2004). Bacillus megaterium - a versatile tool for production and secretion of heterologous proteins. 3rd Recombinant Protein Production Meeting, Portugal, Spain.

2003

Malten, M., Barg, H., Martens, J. H., Biedendiek, R., Palme, O., Mooser, J., Künkel, A., Jahn, D. (2003). Bacillus megaterium - a tool for biotechnology VAAM-Jahrestagung, Berlin, Germany.

2002

Malten, M., Jahn, D. (2002). Production and secretion of dextransucrase DsrS in Bacillus megaterium. Biocatalysis in the Food and Drink Industries, Society for Chemical Industry, London, Great Britain

Patente/Patents

Malten, M., Jahn, D. (2003). Produktion und Sekretion von Glucosyltransferasen. Patent DE 102 25 380.

Barg, H., Künkel, A., Jahn, D. (2003). Weitere verbesserte Verfahren zur Herstellung von Vitamin B12 mit Bacillus megaterium Patent angemeldet.

Barg, H., Warren, M. J., Künkel, A., Jahn, D. (2002). Verbessertes Verfahren zur Herstellung von Vitamin B12 mit Bacillus megaterium. Patent DE 10150323.7.

Barg, H., Martens, J.-H., Warren, M. J., Künkel, A., Jahn, D. (2001). Optimierte Verfahren zur Herstellung von Vitamin B12 mit Bacillus megaterium. Patent DE 10141132.4.

Martens, J.-H., Barg, H., Warren, M. J., Künkel, A., Jahn, D. (2001). Verfahren zur Herstellung von Vitamin B12 mit Bacillus megaterium. Patent DE 10141131.6.

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